WebFITC, green fluorescence (λ Ex = 506 nm, λ Em = 529 nm) Rhodamine B, orange fluorescence (λ Ex = 560 nm, λ Em = 584 nm) Nile Blue A, red fluorescence (λ Ex = 636 nm, λ Em = 686 nm). Outstanding characteristics of fluorescent MF particles (Figure 2) are their narrow size distribution and an intense color and fluorescence. The homogeneously ... WebFluorescein (resorcinolphthalein) is a synthetic organic photoactive dye compound, first synthesized by von Bayer in 1871 [1]. Some of the advantageous features of fluorescein fluorophores are good water solubility and absorption, emission, and maximum brightness at physiological pH (Anthoni et al., 1995) [2].

Fluorescent dyes Miltenyi Biotec Sverige

WebFluorescein is also known as a color additive (D&C Yellow no. 7). The disodium salt form of fluorescein is known as uranine or D&C Yellow no. 8. ... Fluorescein has a pK a of 6.4, and its ionization equilibrium leads to … WebJan 10, 2024 · For example, Alexa Fluor®488, one of the most commonly used dyes, has an excitation maximum at 493 nm, which allows excitation with a standard 488 nm laser, … on ramp training

Fluorescent Microparticles and Nanobeads - Sigma-Aldrich

WebFluorescein isothiocyanate (FITC) is currently the most commonly used fluorescent dye for flow cytometry analysis. When excited at 488 nanometers, FITC has a green emission that is usually collected at 530 nanometers, the FL1 detector of a FACSCalibur. WebThe fluorescence from FITC-PEG-lipid exhibited a main peak at 495 nm and a shoulder peak at 465 nm, and showed a clear pH-dependence. To validate our ratiometric confocal laser scanning microscopy ... onr and associates