High-throughput screens for eef-2 kinase

WebeEF-2 kinase is a highly conserved protein kinase in the calmodulin -mediated signaling pathway that links multiple up-stream signals to the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 ( EEF2) and thus inhibits the EEF2 function. [6] [8] Activation [ edit] WebFeb 14, 2024 · To identify kinases that would regulate brown adipocyte differentiation and formation, we developed a high-content and high-throughput in vitro image-based screening system that combined two experimental approaches (fig. S1A).

Development of a Cell-Based, High-Throughput Screening Assay …

WebThese yeast-based methods range from HTP screens to identify novel hits against promising parasite kinase targets to the identification of potential antiparasitic kinase inhibitors extracted from databases of yeast chemical genetic screens. Keywords: Drug discovery; Drug screening; High-throughput; Kinase inhibitors; Neglected diseases; Yeast. WebAug 5, 2015 · High-throughput screens for eEF-2 kinase. Journal of Biomolecular Screening 19: 445–452. Article CAS PubMed Google Scholar Drews, J. 2000. Drug discovery: A historical perspective. Science 287: 1960–1964. Article CAS PubMed Google Scholar Du, Y. 2015. Fluorescence polarization assay to quantify protein-protein interactions in an HTS … chinook baseball schedule https://shafersbusservices.com

Eukaryotic elongation factor 2 kinase, an unusual ... - ScienceDirect

WebSep 27, 2013 · eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of high-throughput assay systems may expedite the process. Two high-throughput assays are described for eEF-2K using recombinant, tag-free enzyme purified from bacteria. WebAug 24, 2024 · The High-throughput screening method screens millions of chemical and biological compounds in a short interval of time. It is an automated process and screens many biological or chemical compounds for their therapeutic potential. ... As an example, the high-throughput screening process successfully identified a potent pan-SRC kinase … WebJun 10, 2014 · High-throughput screening platforms have proved beneficial for protein kinase drug discovery and development, as large compound libraries can be screened with ease and compound potency and selectivity across a subset of the kinome can be determined [13], [14]. granite view apartments

Development of a 384-well colorimetric assay to quantify …

Category:Design and Implementation of High Throughput Screening Assays …

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High-throughput screens for eef-2 kinase

Eukaryotic elongation factor 2 kinase as a drug target in cancer, …

WebHigh-throughput screens for eEF-2 kinase (Q35002039) From Wikidata. Jump to navigation Jump to search. scientific article. edit. Language Label Description Also known as; English: High-throughput screens for eEF-2 kinase. scientific article. Statements. instance of. scholarly article. 1 reference. stated in. WebMay 20, 2024 · Background: Eukaryotic elongation factor 2 kinase (eEF2K) regulates the elongation stage of protein synthesis by phosphorylating eEF2, a process related to various diseases including cancer and cardiovascular and neurodegenerative diseases. In this study, we describe the identification of novel eEF2K inhibitors using high-throughput screening …

High-throughput screens for eef-2 kinase

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WebNov 26, 2007 · Kinases are important drug discovery targets for a wide variety of therapeutic indications; consequently, the measurement of kinase activity remains a common high-throughput screening (HTS) application. Recently, enzyme-coupled luciferase-kinase (LK) format assays have been introduced. WebSep 27, 2013 · eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of …

WebHere, we describe the purification of the protein kinase, eEF-2K, and the utilization of three biochemical assays in the course of identifying small molecules that block its enzymatic … WebJul 15, 2014 · Eukaryotic elongation factor-2 kinase (eEF2K), encoded by the EEF2K gene, is well-known to be a Ca 2+ /calmodulin (CaM)-dependent kinase which can negatively modulate protein synthesis. It is highly conserved among eukaryotes from mammals to invertebrates, of which human and mouse may have 99 % overall amino acid identity.

WebMay 1, 2014 · eEF-2 kinase dictates cross-talk between autophagy and apoptosis induced by Akt Inhibition, thereby modulating cytotoxicity of novel Akt inhibitor MK-2206. ... High-throughput screens for eEF-2 kinase. J Biomol Screen, 19 (2013), pp. 445-452. Google Scholar. Diggle et al., 1998. WebEvidence that elongation factor 2 kinase (eEF-2K) has potential as a target for anticancer therapy and possibly for the treatment of depression is emerging. Here the steady-state …

WebSep 27, 2013 · eEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of …

WebMay 1, 2016 · Eukaryotic elongation factor 2 kinase (eEF2K) inhibitors may aid in the development of new therapeutic agents to combat cancer. Purified human eEF2K was … granite versus formica countertopsWebMay 1, 2014 · The activity of eEF-2, the main eEF involved in the translocation step of elongation, can be inhibited by the eukaryotic elongation factor 2 kinase (eEF2K) (Ma, 2024). eEF2K is an... graniteville schoolWebWe report here the development and optimization of a simple 384-well colorimetric assay to measure H(2)O(2) generated by the redox cycling of compounds incubated with reducing agents in high-throughput screening (HTS) assay buffers. The phenol red-horseradish peroxidase (HRP) assay readily detected H(2)O(2) either added exogenously or generated … chinook baseball wigraniteville sc hotelsWebeEF-2 kinase is a potential therapeutic target for breast cancer, gliomas, and depression. No potent inhibitors of eEF-2K have been reported, and thus development of high-throughput … chinook bases ukWebIntroduced, optimized and conducted an in vitro high-throughput drug screen assay for compounds with activity against liver stage malaria. Discovered and confirmed several compounds active against ... chinook baseball leagueWebJan 24, 2014 · Using this high-throughput assay, we screened more than 7000 compounds and discovered additional small molecules that inhibit the ATM kinase and further validated these hits by secondary assays. Introduction chinook baseball bonney lake